岁岁又重阳

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岁岁又重阳范文第1篇

【摘要】

目的：检测视网膜色素上皮细胞对骨髓间充质干细胞分化的调节作用。方法：体外培养人骨髓间充质干细胞（BMSC）和视网膜色素上皮（RPE）细胞。RPE细胞采用紫外线照射处理，而后与CFSE标记的BMSC共培养14d。并在共培养体系中加入牛眼视网膜提取物（BRE）以研究视网膜成分对此分化过程的影响。采用NSE， Nestin和GFAP抗体标记检测BMSC分化前后的表达特征。结果：BMSC在与RPE细胞共培养后，能够分化成为神经样细胞，并表达神经性细胞的特异性标记NSE，Nestin和GFAP。BRE能够显著促进共培养体系中BMSC向神经样细胞的分化。结论：RPE和BRE能够诱导BMSC分化为神经样细胞。

【关键词】 髓间充质干细胞；视网膜色素上皮细胞；神经样细胞；牛眼视网膜提取物

AbstractAIM: To detect the differentiation effects of retinal cells or extracts on bone marrowderived mesenchymal stem cells (BMSC).METHODS: Human fetal BMSC were previously labeled by carboxyfluorescein succinimidyl ester (CFSE)， and cocultured with retinal pigment epithelial （RPE） cells which were pretreated with ultraviolet irradiation at a ratio of 1∶1 to induce the differentiation of BMSC for up to 14 days. In some assays， a retinal extract of bovine retinal extract (BRE) was added to detect the potential effects of retinal component on the differentiation of BMSC. In addition， neuronspecific enolase (NSE)， Nestin and Glial fibrillary acidic protein (GFAP) immunostaining were performed to determine the characteristics of BMSC.RESULTS: The results indicated that by cocultured with RPE cells， fetal BMSC were differentiated into neurallike cells expressing special neuronal markers Nestin， GFAP and NSE. And the expression of these markers was obviously increased by BRE. CONCLUSION: Retina derived cells and extracts can induce the differentiation of BMSC into neurallike cells.

KEYWORDS: bone marrowderived mesenchymal stem cells; retinal pigment epithelial cells; neurallike cells; bovine retinal extract

INTRODUCTION

According to the origin and potential of differentiation， two types of stem cells can be distinguished: embryonic stem cells (ESC) and somatic stem cells (SSC). SSC are isolated from fetal (after gastrulation) or adult tissues， but classically the differentiation fate of these cells are believed to be limited to the cell types that belong to the tissue from which they originate. However， previous studies have suggested that these tissuespecific stem cells might be able to differentiate into cell types of other lineages[1]. Bone marrowderived mesenchymal stem cells (BMSC) are one of the major subpopulations of SSC， which is extensively studied with respect to transdifferentiation potential[2]. Recent studies have described that BMSC can be differentiated into neurallike cells in vitro under specific induced culture conditions[3，4] or developed spontaneously differentiation under noninduced standard culture conditions[57]. Usually the differentiation is induced just by addition of growth factors and/or chemicals in culture medium. The agents include βmercaptoethanol and dimethylsulfoxide， epidermal growth factor (EGF) and brainderived neurotrophic factor (BDNF)， isobutylmethylxanthine/dibutyryl cAMP， or 5AzaC/growth factors. Moreover， a small proportion of BMSCderived cells differentiated into neuronlike cells expressing NeuN and glial cells expressing Glial fibrillary acidic protein (GFAP) when cocultured with rat fetal mesencephalic or striatal cells. The retinal pigment epithelium(RPE) is a hexagonally packed monolayer cell in ocular retina that performs critical functions in the maintenance of the physiology of photoreceptors which is developed from the outer layer of the optic cup. The research of Chiou et al[8] shows that coculture of human BMSC with human retinal pigment epithelium (HRPE) cells facilitates the retina and photoreceptorlineage differentiation of adult human BMSC. This system indicates the retinal differentiation potential of BMSC. In this study， we established a coculture fetal BMSC/RPE system by directly mixing them and showed that under such condition， fetal BMSC was differentiated into neural phenotypelike cells and expressed neuronal markers such as Nestin， GFAP and neural specific enolase[5]. Besides， our results demonstrated that bovine retinal extract (BRE) could markedly promoted the neurallineage differentiation of fetal BMSC in vitro.

MATERIALS AND METHODS

Materials Human fetal BMSC were cultured in this assay. The use of human tissues in this study was approved by the Shandong Eye Institute Medical Ethics Committee and was in compliance with the Declaration of Helsinki. In order to eliminate unwanted types of cells presented in the marrow aspiration， mononuclear cells were obtained by Ficoll Hypaque density gradient centrifugation (lymphoprep， 1073g/L; TBD， China) and BMSC were selected by plastic adhesion. Briefly， a small percentage of cells isolated from the density interface of 1073g/L were seeded in 6 well plates using the medium of LG DMEM supplemented with 100mL/L FBS (Gibco， USA). After 3 days undisturbed to promote cell attachment， the nonadherent cells were removed by changing the medium. At nearconfluence， about two weeks after initial plating， cells were subcultured after trypsin digestion. Fetal BMSC were isolated by Ficoll Hypaque density gradient centrifugation and the physical property of adherence to plastic culture dishes were confirmed. BMSC cultured in our experiment grew as fibroblastic cells with scant cytoplasm and contain granules around nuclei， which were similar to the cells described in previous reports[8]. Fetal RPE cells were isolated and cultured as described previously[9] and these cells showed typical polygonal shape. Briefly， sheets of RPE were dissected from the choroids of fetal eye cups and cultured in lowcalcium (0.05mmol/L Ca2+) MEM (Sigma， St. Louis， MO; catalog number M8028) medium containing proposed supplements according to the reports of Hu and Bok [9]. Floating RPE cells were collected and passaged with normalcalcium medium (Sigma， St. Louis， MO; catalog number M2279) containing the same supplements.

BMSC/RPE 　coculture Firstly， fetal BMSC at 35 passage were labeled with CFSE (PKCA705C375， Probior GmbH) in order to be distinguished from RPE cells. A total of 106 to 107 cells were washed twice with phosphate buffered saline (PBS) and then incubated with 2.5μmol/L CFSE in PBS for 10 minutes in dark at room temperature. Then cells were washed twice with media containing 100mL/L FBS. Secondly， the second passage of fetal RPE cells were pretreated under a UV lamp for 15 minutes to abolish its selfroliferation as the method of Chiou et al[8] and washed three times with DHanks. Then the inactivated cells were digested with 2.5g/L trypsin0.2g/L EDTA and resuspended in medium containing 100mL/L FBS. Lastly， 2×105 of fetal BMSC and RPE cells prepared as above were mixed together and plated on gelatincoated 24wells plates for up to 14 days. In some assays， 10μg BRE was added in the medium. BRE was prepared by homogenizing 12 fresh bovine retinas per 100mL CMFBSS and stirring overnight in the dark at 4℃. The mixture was cleared by centrifugation (12000g for 20 minutes) and the supernatants were collected[9]. The noninduced fetal BMSC were cultured as the control group. In each group four duplicate wells were set up， and the assay was repeated for 3 times independently.

Immunocytochemistry Cells were fixed at 20℃ with cold methanol for 5 minutes. For staining， samples were rehydrated in PBS/2mL/L Triton X100. Nonspecific binding was blocked with 100mL/L normal serum in PBS/2mL/L Triton X100 for 30 minutes at room temperature. Cells were then incubated overnight at 4℃ with the following primary antibodies diluted in blocking solution， polyclonal rabbit antinestin (1∶100， Boster， China); polyclonal rabbit antiNSE (1∶100， Boster， China); polyclonal rabbit antiGFAP (ready to use， Beijing Zhongshan Golden Bridge Biotechnology CO， LTD， China). Rhodamine conjugated Goat anti rabbit IgG (1∶100; Beijing Zhongshan Golden Bridge Biotechnology CO， LTD， China) was used as secondary antibody. After washing with PBS for 3 times， samples were counterstained with DAPI (Santa Cruz， USA). Negative controls were performed by omitting primary antibodies.

RESULTS

Differentiation of BMSC In this assay， we detected the differentiation characteristics of BMSC cocultured with inactivated RPE cells. In order to distinguish BMSC from RPE cells， BMSC were labeled with CFSE before coculture. After 3 days， we observed that some of cytoplasm of cultured cells retracted toward the nucleus， presenting a more spherical shape and extending processes; the changed cells were CFSEpositive demonstrating the BMSC origin(Figure 1). Furthermore， the cocultured BMSC aggregated into neurospherelike body and cells with a spindleshaped morphology were observed 10 days later (Figure 2). Immunocytochemistry was performed to investigate the expression of neuronal markers， including Nestin (neural precursor marker)， NSE (neuronal marker) ， and GFAP (astroglial maker). These analysis revealed that BMSC expressed the neural makers when cocultured with RPE. On the contrary， no neural marker was found in untreated fetal BMSC (Figure 3).Figure 1The morphological changes of fetal BMSC after induced for 3 days A and D， BMSC cocultured with RPE cells without addition of BRE; B and E， BMSC cocultured with RPE cells with addition of BRE; C， untreated BMSC. A， B and C， phasecontrast photomicrographs; D and E， confocal photomicrographs presenting the CFSEstaining cells， ×100 （略）Figure 2The morphological changes of fetal BMSC after induced for 10 days A and D， BMSC cocultured with RPE cells without addition of BRE; B and E， BMSC cocultured with RPE cells addition of BRE; C， untreated BMSC. A， B and C， phasecontrast photomicrographs; D and E， confocal photomicrographs presenting the CFSEstaining cells， ×100 （略）Figure 3Nestin， NSE and GFAP expression of BMSC upregulated in BRE treated group after induced for 14 days(Bar=100μm)A， D and G， +RPE cells， BRE; B， E and H， +RPE cells +BRE; C， F and I， untreated（略）

Effects of BRE In some assay， 10μg BRE was added to the induction system. The neuronlike morphology of BMSC treated with BRE in the medium was induced much more markedly at the early induction stage (Figure 1). CFSE is used to fluorescently label live cells and is equally partitioned to daughter cells during pision and can be used to measure cell proliferation. After 10 days， the CFSE intensity of the cells was markedly decreased by addition of BRE in culture medium(Figure 2)， suggesting that BRE may promote the proliferation of the cells. The immunoreactivity for specific neural markers was increased obviously (Figure 3). Especially， the expression of NSE and GFAP was significantly upregulated as compared with the group without BRE.

DISCUSSION

In this study， we detected the effects of RPE cells and BRE on the differentiation of BMSC to determine the potential induction function of retinaderived components on BMSC. Before induction， fetal BMSC had a flat， elongated， spindlelike structure， similar to that of fibroblasts. After induction， the CFSE positive BMSC showed the morphological characteristics of neuronal cells such as long multipolar extensions and branching ends after 3 days; and then aggregated into spheroid 10 days later. And the neural lineage differentiation of BMSC was demonstrated by the expression of some specific neural markers detected by immunocytochemistry assay. In addition， BRE could promote the neurallike cells differentiation of BMSC in this coculture system. We didnt get the similar results of differentiation BMSC into retinal lineage cells as Chiou et al by coculture of BMSC with RPE cells in this experiment. This may be attributed to the existence of some differences between our induction methods. Chiou et al[8] first induced the BMSC to a spheroid body using neurogenic selection medium for 2 weeks， and then followed a further differentiation in the medium containing RPE cells as a feeder layer for another 23 weeks. However， we directly combined the undifferentiated BMSC and RPE cells together and induced differentiation for 2 weeks. Considering the neural ectoderm developing origin of RPE， RPE cells may play a role in inducing BMSC into neurallineage cells.

Previous reports seemed that the protein of BRE has the function of maintenance the proliferation and differentiation of RPE cells[9]. In order to detect whether BRE could have some function in BMSC differentiation， 10μg protein/mL of BRE was used. The results revealed that BRE could promote a higher expression of nestin， an intermediate filament protein that is predominantly expressed during neural development to some degree[10]. Especially， BRE also enhanced the expression of NSE， a unique form of the glycolytic enzyme enolase found in neurons and in virtually all of the neuroendocrine， paraneuronal cell types， and GFAP， a glial protein that is found in glial cells such as astrocytes. These results indicated that BRE could promote the neural differentiation of BMSC. Given that the retina is developed from neural ectoderm and considering that BRE contains prominent portion of intracellular proteins which do not release and contact cell during retinal development; our results suggested that some of the retinal component must have functions in inducing the differentiation of neural lineage cells. Interesting， these neurallike cells differentiated from BMSC in this assay are both NSE and GFAP positive， we suppose that these neurallike cells are neuron/astrocyte precursor cells which may further differentiated into neuron or astrocyte cells. In conclusion， BMSC from fetal bone marrow were differentiated into neurallike cells expressing the special markers of neural cells by coculture with RPE cells， and the component from retinal may promote BMSC changing into neural lineage cells.

参考文献

1 Hughes S. Cardiac stem cells. J Pathol 2002; 197(4): 468478

2 Jiang TS， Cai L， Hui YN， Yan F. Study on transdifferentiation of rats mesenchymal stem cells into corneal epithelial cells in vitro. Int J Ophthalmol(Guoji Yanke Zazhi) 2007; 7(2): 339341

3 Deng W， Obrocka M， Fischer I， Prockop DJ. In vitro differentiation of human marrow stromal cells into early progenitors of neural cells by conditions that increase intracellular cyclic AMP. Biochem Biophys Res Commun2001;282(1):148152

4 Kim BJ， Seo JH， Bubien JK， Oh YS. Differentiation of adult bone marrow stem cells into neuroprogenitor cells in vitro. Neuroreport 2002;13(9):11851188

5 Deng J， Petersen BE， Steindler DA， Jorgensen ML， Laywell ED. Mesenchymal stem cells spontaneously express neural proteins in culture and are neurogenic after　transplantation. Stem Cells 2006;24(4):10541064

6 Lamoury FM， CroitoruLamoury J， Brew BJ. Undifferentiated mouse mesenchymal stem cells spontaneously express neural and stem cell markers Oct4 and Rex1.Cytotherapy 2006;8(3):228242

7 Tseng PY， Chen CJ， Sheu CC， Yu CW， Huang YS. Spontaneous differentiation of adult rat marrow stromal cells in a longterm culture. J Vet Med Sci 2007;69(2):95102

8 Chiou SH， Kao CL， Peng CH， Chen SJ， Tarng YW， Ku HH， Chen YC， Shyr YM， Liu RS， Hsu CJ， Yang DM， Hsu WM， Kuo CD， Lee CH. A novel in vitro retinal differentiation model by coculturing adult human bone marrow stem cells with retinal pigmented epithelium cells. Biochem Biophys Res Commun 2005;326(3):578585

岁岁又重阳范文第2篇

【摘要】 目的 观察血管紧张素Ⅱ联合5-氮杂胞苷体外诱导骨髓间充质干细胞 (BMMSCs) 定向分化为心肌样细胞的作用。方法 采用密度梯度离心法分离大鼠BMMSCs，取第3代BMMSCs进行分组诱导:血管紧张素Ⅱ组(AngⅡ，终浓度为0.1μmol/L)、5-氮胞苷组 (5-aza，终浓度为10μmol/L)、AngⅡ联合 5-aza组 (终浓度分别为0.1μmol/L与10μmol/L)、空白对照组。诱导24h后更换常规培养液继续培养 4 周。倒置相差显微镜观察细胞的形态学变化，MTT法检测细胞活性及增殖能力，免疫荧光染色法鉴定诱导后BMMSCs中心肌特异性肌钙蛋白 I (cTnI) 的表达，流式细胞计数法计算心肌样细胞诱导率， 透射电镜观察诱导后心肌样细胞的超微结构。结果 原代培养的BMMSCs14天形成集落， 传代细胞体积变大， 诱导后细胞呈长梭形，呈一致性生长， 并出现肌岛样结构。MTT法显示AngⅡ联合 5-aza组细胞增殖能力优于AngⅡ组与5-氮胞苷组。免疫荧光染色结果显示诱导后BMMSCs 表达心肌特异性蛋白cTnI。流式细胞计数法显示：AngⅡ组、5-aza 、AngⅡ联合 5-aza组心肌样细胞诱导率分别为(25.3±2.2)%、(24.6±1.9)%、(30.0±1.7)%，AngⅡ联合 5-aza组心肌样细胞诱导率高于AngⅡ组与5-氮胞苷组(P>0.05)。透射电镜可见平行排列的肌丝、Z线样物质。结论 血管紧张素Ⅱ联合5-氮杂胞苷可在体外诱导大鼠BMMSCs定向分化为心肌样细胞，其诱导分化率高于单纯5-氮杂胞苷诱导。

【关键词】 血管紧张素Ⅱ; 5-氮杂胞苷; 骨髓间充质干细胞;心肌样细胞

[Abstract] Objective To observe the effect of the differentiation of bone marrow mesenchymal stem cells(BMMSCs) into cardiomyocyte-like cells with 5-azacytidine and angiotensinⅡ.Methods BMMSCs were isolated from bone marrow of SD mouse by density gradient centrifugation. The third passage cells were pided into four groups:AngⅡgroup (0.1μmol/ L)，5-aza group(10μmol/L)，AngⅡ combined with 5-aza group (0.1μmol/L and 10μmol/L)，untreated group as control. After 24h induction， the medium was changed to complete culture medium without any inductor and the cells were culture for 4 weeks. The morphological changes were observed under phase contrast microscope. The effect of AngⅡ and 5-aza on the BMMSCs proliferation was observed with methyl thiazolyl tetrazolium (MTT) assay. The cardiomyogenic cells were identified by immunofluorescence staining. The differentiation ratio was examined by flow cytometer. The ultrastructures of the induced cells were viewed with a transmission electron microscope.Results BMMSCs of primary culture formed cell colonies at 14 days. The passaged cells were larger than those of primary culture. After induction， the cells presented long spindle， aligned in parallel and formed “muscle island”- like structure. MTT assay showed that the cell proliferation in AngⅡ and 5-aza group outweighed that of in AngⅡgroup or 5-aza group. The expression of specific protein of cardiac troponin I (cTnI) in induced BMMSCs was positive. Flow cytometer showed that the differentiation ratio in AngⅡgroup，5-aza group and AngⅡ combined with 5-aza group were respectively(25.3±2.2)%，(24.6±1.9)%，(30.0 ±1.7)%， demonstrating that the differentiation ratio in AngⅡ combined with 5-aza group was higher than that of in AngⅡ group or 5-aza group(P>0.05). Transmission electron microscopy showed that the induced cells had myofilaments， Z line-like substances.Conclusion AngiotensinⅡ combined with 5-Azacytidine can induce the differentiation of BMMSCs into cardiomyocyte-like cells， and the differentiation ratio was higher than that of in 5-aza only.

[Key words] angiotensin Ⅱ;5-azacytidine;bone marrow mesenchymal stem cells;cardiomyocyte-like cells

缺血性心脏病在我国的发病率逐年升高，严重威胁着人类的生存及生活质量。近几年来，细胞移植技术发展迅速，借助此技术可以在坏死部位移植具有收缩功能的细胞，为治疗缺血性心脏病提供了新的方法，也成为治疗缺血性心脏病的研究热点课题。随着研究的深入，选用的靶细胞目前多集中于有多向分化能力的各种干细胞，其中研究的较多的是骨髓间充质干细胞(bone marrow mesenchymal stem cells， BMMSCs)[1]，有研究显示BMMSCs可以被化学物质 5-氮杂胞苷 (5-azacytidine， 5-aza)诱导为心肌样细胞[2，3]。也有研究显示，血管紧张素Ⅱ在体外可能经细胞外信号调节激酶通路诱导人BMMSCs向心肌样细胞分化[4]，提示血管紧张素Ⅱ对BMMSCs的分化发挥重要作用。于是我们设想：血管紧张素Ⅱ联合5-氮杂胞苷共同诱导能否提高BMMSCs向心肌细胞的分化率 ?与单纯血管紧张素Ⅱ及单纯 5-aza的诱导作用相比又有何差别?本实验即通过联合诱导培养，观察血管紧张素Ⅱ联合5-氮杂胞苷对 BMMSCs向心肌细胞分化所起的作用，并与传统诱导分化剂 5-aza比较。

1 材料与方法

1.1 主要试剂与实验动物 AngⅡ ( Sigma， USA)， 5-azacytidine ( Sigma， USA)，L-DMEM 培养基(Hyclone， USA)，胎牛血清(杭州四季青)， 胰蛋白酶(Sigma， USA)， Ficoll 淋巴细胞分离液(1.077g/ml，TBD公司)， MTT (Sigma，USA)，DMSO (Sigma，USA)，羊抗鼠cTnI抗体(Santa cruz)，FITC标记的抗羊IgG (Boster)，Hoechst33258 (Sigma， USA)。健康SD 大鼠，4周龄，质量80～100g，雄性，由第四军医大学动物实验中心提供。

1.2 方法

1.2.1 BMMSCs的体外分离和培养 颈椎脱臼法处死大鼠，取其四肢长骨，75%酒精浸泡5min。无菌条件下分离出胫骨和股骨，剪除两端骨骺，用不含血清的DMEM培养液冲洗骨髓腔，充分混匀。将细胞悬液缓慢加入含淋巴细胞分离液的离心管中，2000r/min， 离心20min。取中间云雾状有核细胞层， 加入L-DMEM不完全培养液，充分混匀后，1500r/min， 离心10min，弃上清，加入完全培养液，充分混匀后，调整细胞密度为1×106/ml，接种于T-25塑料培养瓶， 37℃含体积分数为0.05的CO2孵箱中饱和湿度培养。3天后首次换液， 除去不贴壁的悬浮细胞，以后每3天换液1次。待细胞长到80%融合时， 用2.5g/L胰蛋白酶进行消化，按1：2的比例进行传代。体外培养至第3代备用。

1.2.2 BMMSCs的诱导分化 将传至第3代的BMMSCs进行分组诱导，共分为4组：(1)AngⅡ组(终浓度为0.1μmol/L);(2)5-aza组(终浓度为10μmol/L);(3)AngⅡ加5-aza组(终浓度分别为0.1μmol/L、10μmol/L);(4)空白对照组， 仅用基本培养基诱导。各组诱导24h后更换完全培养基继续培养，每3天换液1次， 并观察细胞形态变化， 连续培养4周。

1.2.3 形态学鉴定 每日在倒置显微镜下观察诱导前、后细胞生长和形态变化。

1.2.4 MTT法检测细胞活性并描绘细胞增殖曲线 将第3代BMMSC以1×105/ml接种于4个96孔板中， 每块板选取24孔，分为4组，每组6孔，每孔加200μl细胞悬液。培养24h后吸弃孔内培养液，PBS洗涤2次，前3组分别加入含AngⅡ、5-aza、AngⅡ和5-aza的诱导培养液，第4组加入基本培养基作为对照组。诱导24h后各组更换完全培养基继续培养。于培养 1、3、5、7天后进行MTT 检测，每孔加入20μl MTT(5mg/ml)，孵育4h后终止培养。弃孔内上清液，每孔再加入150μlDMSO， 打匀振荡10min，使结晶物充分溶解。选择490nm波长，在酶联免疫检测仪上测定各孔光吸收值(A)，并描绘细胞增殖曲线。

1.2.5 免疫荧光细胞化学检测 将诱导培养1、2、3、4 周的细胞进行爬片，4%的多聚甲醛固定，3%过氧化氢-甲醇室温孵育10min ，正常山羊血清封闭，加cTnI抗体，4℃过夜，PBS冲洗，加FITC标记的二抗，37℃孵育40min，PBS 冲洗，Hoechst33258室温孵育10min，PBS 冲洗，甘油封片，荧光显微镜观察并拍照。

1.2.6 流式细胞计数法 2.5g/L的胰蛋白酶消化收集诱导4周的细胞， PBS调整细胞悬液密度约1×106个细胞 /ml， 滴加羊抗鼠 cTnI抗体，室温 1h，再滴加 FITC标记的抗羊 IgG，室温避光 40min，40g/L多聚甲醛固定 20min。最后流式细胞仪测定 FITC阳性标记细胞占细胞总数百分比。

岁岁又重阳范文第3篇

重阳节，又称重九节、晒秋节、踏秋，中国传统节日。庆祝重阳节一般会包括出游赏秋、登高远眺、观赏、遍插茱萸、吃重阳糕、饮酒等活动。每年的农历九月初九日，与除夕、清明节、中元节三节统称中国传统四大祭祖的节日。重阳节，早在战国时期就已经形成，到了唐代被正式定为民间的节日，此后历朝历代沿袭至今。重阳与三月初三日踏春皆是家族倾室而出，重阳这天所有亲人都要一起登高避灾。

《易经》中把六定为阴数，把九定为阳数，九月九日，日月并阳，两九相重，故曰重阳，也叫重九。重阳节早在战国时期就已经形成，自魏晋重阳气氛日渐浓郁，倍受历代文人墨客吟咏，到了唐代被正式定为民间的节日，此后历朝历代沿袭至今。

1989年农历九月九日被定为老人节，倡导全社会树立尊老、敬老、爱老、助老的风气。2006年5月20日，重阳节被国务院列入首批国家级非物质文化遗产名录。 2016重阳节经典贺词

1.送你九九重阳节开心秘方：工作放一放，心事搁一搁，烦恼抛一抛，身体歇一歇，心情酿一酿，开心笑一笑，牙齿露一露，快乐好心情。祝天天开心!

2.岁岁重阳，今又重阳，日日思念，来把情传，重阳九月九，友谊就如酒，永远都会久，重阳登高日，祝福送给你，愿你重阳佳节日，开心无限期!九九重阳节祝福语

3.岁岁重阳，今又重阳，傲霜满地香;久久思量，久久难忘，九九重阳胜春光;美酒一杯，秋雁两行，福泽深厚又绵长。祝重阳快乐，永远健康!

4.岁岁重阳，今又重阳，九九相逢祥瑞降。不似春光，胜似春光，秋高气爽喜洋洋。金菊飘香，归雁成行，健康喜乐福绵长。重阳节，祝你幸福安康!

5.松柏不残四季翠，山村难老百岁人，三三令节春时松，更高九九芳辰重阳鹤添寿，愿秋风捎去我的思念和祝福，祝你越活越精神，越活越年轻!

6.思念在岁月中荡漾，南飞的大雁披上了霓裳，金菊在清风中绽放，转眼又到了重阳。看一回天高云淡，走一路山高水长，道一声幸福安康。重阳快乐!

7.说重阳，道重阳，收获季节，遍地金黄;秋风吹动，秋高气爽，稻子熟透，渐香，岁月岁岁重阳，今又重阳，不是春光，胜似春光，一根扁担，两挑箩筐，三秋雁阵，四分五行，六六大顺，七下八上，九九重阳，十月农忙，短信在此，祝你健康!

8.虽然我不能陪你品酒赏菊，但我们可以共赏同一条短信;虽然我不能陪你登高插萸，但我们可以转发同一条祝福;重阳节，总有一条短信专门送给你。

9.送一缕阳光给你，在秋日的萧瑟中为你捎去温暖，送一份祝福给你，在重阳佳节即将到来的日子里为你带来关怀，预祝重阳节快乐，天天快乐。

10.送上一支山茱萸，愿你永远愉快;送上一块重阳糕，愿你永远高兴;送上一杯酒，愿你幸福久久。重阳节到了，祝福你节日天天好心情!

11.送你清风让你心旷神怡，送你细雨洗去你疲惫的汗迹，让蓝天作封白云作信，让流星作我的特快专递，送你彩虹通向梦想之旅，祝你重阳节快乐!

12.如歌，天地变凉，思念不变，祝福奉上。重阳节快乐!

13.霜降遇重阳送你养身小方子，没事洗个鸭梨子，润润小嗓子，睡觉盖好了被子，赶走疾病的引子，打着问候的旗子，送来好运的影子，祝你快乐过日子!

14.双九重阳佳节到，短信祝福来问好：相逢总是很奇妙，生活无忧无烦恼，工作压力瞬间消，伤心失落全跑掉，锻炼身体多散步，愿你重阳更美好!2013重阳节祝词

15.双九节，出游赏景好时节;九九节，登高远眺值佳节;重阳节，观赏好季节;遍插茱萸、吃重阳糕、饮酒，可去邪，祝君重阳过好节!

16.帅哥味好纯：但得夕阳无限好，何须惆怅近黄昏，愿你拥有一个美好、快乐的节日!秋风徐徐，重阳九九，蒸上九重的粟糕，备好香醇的菊酒，等着与你分享。

17.岁岁重阳，今又重阳，又见香;不是春光，胜似春光，秋色迷人心芬芳。让我登上高处，为你送来真诚和爱的祝福：祝重阳快乐，合家康泰!

18.岁岁重阳，今又重阳，谁道秋日凉?重阳节里歌声放，祝福飞来心飞扬。九月九里共举杯，声声祝福暖心扉。短信送到你这里，祝福也能化伤悲!

岁岁又重阳范文第4篇

2、我轻呵出一团气，将满天飘舞的思绪，凝结成一朵白云，用心把它绘的五彩斑斓，载上沉淀的祝福，带去来自远方的重阳节的问候——送给永远开心的你!

3、岁岁重阳，今又重阳，不似春光，胜似春光。松柏不残四季翠，山村难老百岁人。祝您重阳节快乐!

4、佛说：重阳节又到，要相约登高，要常思故友，尤其是现在给你发短信的人!要经常请他吃饭!特别是九月九!要把你最最真诚的祝福都给他!善哉!善哉!

5、回乡的路，曲曲弯弯，辙痕深浅。回家的心，早早晚晚，冲过篱藩。故园的情，岁岁年年，月挂云端。又到重阳倍思亲，异乡客，心相近，祝吾友，多温馨!

6、

7、愿友情像秋日的红叶那样美丽，愿你心情像秋日的蓝天那样爽朗，愿爱情像秋日的果实一样丰硕，愿老朋友重阳节开心快乐!

8、一九二九，天长地久;三九四九，幸福永久;五九六九，福禄长久;七九八九，神交已久;重阳九九，相伴久久!重阳节快乐!

9、九九重阳，因为与“久久”同音，九在数字中又是数，有长久长寿的含意，况且秋季也是一年收获的黄金季节，重阳佳节，寓意深远。祝你重阳节快乐!

10、重阳节到，我向月老许愿让你幸福，他答应;我向财神许愿让你富裕，他点头;我向王母许愿。。。玉帝说：诸神放假过节，干脆祝收到短信的人心想事成吧!

11、您生命的秋天，是枫叶一般的色彩，不是春光胜似春光，时值霜天季节，却格外显得神采奕奕。祝您老重阳节快乐，健康长寿!

12、夜里开机看见，重阳佳节祝福。千万里送来问候，万千言语祝福，温暖你心扉。我的短信飞快，犹如光速传载，了却重阳开心事，赢得佳人美酒归，歌声满天飞!

13、重阳佳节到来，亲朋好友开怀，祝你心情愉快，心事统统放开。九月九，让我们一起登高望远吧，借着醇香的美酒，分享心中的忧愁与快乐，重阳节快乐!

14、九月九，重阳节。梳个鬏，换个装。拜个舅，唱个喏。喝个酒，散个心，叙个旧，念个情。好日子，年年过。重阳节，更快乐。

15、又是一年重阳时，花点小钱祝福你;秋高气爽好时节，登高赏菊最适宜;发封短信问候你，愿你事事都顺利!祝重阳节快乐!

16、九九重阳节，九个祝福送给你，一祝身体好，二祝心情妙，三祝快乐绕，四祝好运到，五祝来钞票，六祝烦恼跑，七祝永不老，八祝万事好，九祝短信告。

17、生活贫穷还是富裕，开心就好;工作辛苦还是轻松，快乐就好;朋友近邻还是远离，惦记就好;祝福简短还是冗长，真心就好!祝你九九重阳快乐一切都好!

18、千重坎万重关，愿快乐阳光伴你灿烂;千重水万重山，愿好运阳光陪在你身边;千重地万重天，愿成功阳光温暖你心间。重阳佳节，愿久久好运把你围绕!

19、九九重阳节，老人最希望健康永久，孩子最盼望快乐长久，情人最期望天长地久，上班族最渴望活力持久，在这个悠久的节日，我最想说：祝你好运更长久!

20、当国庆遇到重阳，这是盛世和传统的碰撞：是要清楚告诉我们，出游欢乐的时光，莫忘家中苍老的爹娘。陪父母登高望远赏菊香，祝愿父母快乐安康，莫忘!

21、九九重阳两九相遇，物极必反，万物凋零。我的祝福经年累月伴随你，不会断绝，不会凋零，永远年轻。重阳节祝你带着快乐迎接秋雨，带着温暖迎接严寒。

22、重阳气坏了灰太狼，不能团聚还要抓羊。对着发毒誓，一定杀尽小绵羊。他正赶着去找你，你要小心的提放。重阳节里多快乐，你这可爱的小绵羊。

23、饮一碗美酒，登一座高山，插一片茱萸，赏一坛，掬一把相思，送一份祝福，又逢重阳佳节，我祝您节日快乐，健康幸福!

24、一年一度重阳，祝福大不一样，生活喜气“阳阳”，职场“阳阳”得意，幸福身边徜“阳”，爱情沐浴阳光，心情阳光灿烂。收下祝福吧!重阳节快乐!

25、重阳节到了，翻遍《辞海》也没有找到一句值得送给你的祝福，所以只能俗气地祝你享的福气如东海泛滥，你领的薪水如南海澎湃。重阳“海皮”!

26、我用彩云编织缱绻的梦境，收集心中每一份感动，许下星空每一个祝愿，记载心头每一丝企盼，交织成一首幸福美丽的乐章，在这重阳佳节伴随你!

27、秋来天气渐转凉，不知不觉到重阳。温馨问候情意长，真诚关怀存心房。秋菊艳艳多欣赏，秋雨潇潇添衣裳。秋风送去我祝福，幸福永伴你身旁。重阳快乐!

28、岁岁重阳，今又重阳，傲霜满地香;久久思量，久久难忘，九九重阳胜春光;美酒一杯，秋雁两行，福泽深厚又绵长。祝重阳快乐，永远健康!

岁岁又重阳范文第5篇

文案一

1、送你一棵茱萸，愿你大吉大利；敬一杯酒，祝你健康又长寿；送你一架云梯，助你登高远望；送你一句祝福，重阳节快乐！

2、有些往事，即使远去也会在心头萦绕；有些人，即使不常联系也会常记在心里；就像你，只轻轻的一个短信，就是我衷心心意的快递！祝：重阳快乐！

3、蓝天作信封，白云作信纸，流星是偶的特快专递。送你清风让你心旷神怡，送你细雨洗去你疲惫的汗迹，送你彩虹通向梦想之旅重阳节！重阳节快乐！

4、九月初九重阳节，遵守传统好风俗。登高远眺金秋景，寓意吉祥步步高。饮酒祈福赏，保佑寿比南山松。尊俗重道度重阳，祝你幸福久久长。

5、朵朵迎风开，悠悠花香沁心脾；九九重阳翩翩至，喜上心头笑开怀；漫步郊外心情爽，徒步登高乐逍遥；举目远眺观美景，心旷神怡爽歪歪；朋友特来送祝福，情谊绵绵深又厚；祝你重阳好心情，生活幸福喜滋滋！

6、朵朵迎风开，悠悠花香沁心脾；九九重阳翩翩至，喜上心头笑开怀；漫步郊外心情爽，徒步登高乐逍遥；举目远眺观美景，心旷神怡爽歪歪；朋友特来送祝福，情意绵绵深又厚；祝你重阳好心情，生活幸福喜滋滋！

7、九九重阳是中国文化中最吉祥的日子，在这金风送爽，桂花飘香，争艳的日子里，我祝各位幸福久久，健康久久，更祝长辈们多福多寿。祝重阳节快乐！

8、送你一盒重阳糕，它以祝福与问候为原料，相间叠放：一层幸福粉，一层平安糖，一层开心豆，一层健康果，愿你重阳节快乐。

9、蓝天作信皮，白云作信纸，流星是我的特快专递。送你清风让你心旷神怡，送你细雨洗去你疲惫的汗迹，送你彩虹通向梦想之旅，祝你重阳节快乐！

10、重庆遇见重阳就是双重，今天祝福与问候双重，祝你工作与生活双重棒，健康与快乐双重好，幸福与甜蜜双重久，此短信你如果收到两遍，那是因为我发重了。祝重阳节快乐！

11、遍地的茱萸都想戴在你头上，长出很多的幸福吉祥，我们一起欢畅疯狂；登到最高处哪怕是你的心上，让金秋的为你飘香，九月九我们的重阳，祝你生活步步高，幸运又安康。

12、月九，送美酒，幸福酒，好运旺，快乐人生乐安康；友谊酒，情意深，久久的祝福长又长；九月九，重阳到，祝你快乐久久！

13、轻轻打开你的手机，慢慢翻看我的祝福：愿今天的你：快乐！今年的你：顺利！今生的你！健康！今世的你：幸福！恭祝您及家人重阳快乐幸福！

14、金秋时节到，空气好又好，九九重阳节，悄悄来到了，登高插茱萸，这个少不了，畅饮酒，这个最为妙，发个短信息，这个最重要，身体要健康，这个我看好。

15、可能你不知道重阳为什么要登高，不知道重阳为什么要插茱萸，不知道重阳为什么要赏，但是我知道发这条短信是为了给你带去真切的祝福！重阳节快乐！

16、我在手机的这边，你在手机的那边，中间是无形的电波，距离阻隔了相见，却阻隔不了祝福，让这条短信随着无形的电波传送到你的手机中，重阳节快乐！

17、九九重阳，好事成双，送给你双重的祝福：快乐+快乐=快快乐乐，圆满+圆满=圆圆满满，长久+长久=长长久久。祝你快快乐乐圆圆满满长长久久。

18、重阳节快乐，一句最简单的祝福送给你，希望你天天都能健康快乐，忘记心中的.烦闷，拥有一份阳光般灿烂的心情。

19、在思念的日子里，留下了如同秋叶般数不尽的美好回忆，我托秋雁把最真心的祝福送给你：愿你在重阳节里美丽如金秋菊，好运如重阳糕，甜美如重阳酒。

20、九月九日上酒楼，酒醉醉酒忘忧愁，久久凝思九九日，九九重阳想舅舅，纠结思念家中舅，久久未见离家久，带酒一瓶送舅舅，祝福永久在心中。

21、重阳节到了，祝你登高望远，事运，财运，爱情运，运运皆高，祝你把酒畅欢，人欢，心欢，情欢，事事皆欢！重阳节快乐！

22、处事秘笈：做人要像李莫愁，刮风下雨都不愁；做事要像张无忌，牛鬼蛇神都不惧；工作要像王重阳，全真七子来帮忙；生活要像韦小宝，快乐幸福没烦恼！

23、稻花香，插茱萸，登高望远喝小酒。吃花糕，又相聚，赏菊品茗会挚友。九月九，重阳日，健康开怀人长久。重阳节即将到来，短信预祝重阳快乐！

24、九九重阳，沉淀着金秋的丰硕，感受了生活的温馨。它是属于花甲卸任转折的轻松、古稀牵手连接的和睦、耄耋拐杖支撑的充实的。一句贴心的问候、一条温馨的短信，祝愿所有朋友节日快乐。

25、重阳节到了，看在我这个这么"重"量级人物在这个"阳"光灿烂的节日里给你祝福问候的份上，你怎么得也该高兴快乐吧！重阳节快乐哦！

26、正值秋高气爽，人寿花香，一条短信带来我的九九的渴望；正值彩云追月，桂花飘香，一条短信带来我的九九的衷肠，祝你开心重阳，幸福重阳！

27、重阳节，更是老人的节日，您生命的秋天，是枫叶一般的色彩，不是春光胜似春光，霜天季节，却格外神采奕奕。祝老人们重阳节快乐，健康长寿！

28、邀你一起品尝九月九的酒，能赏个脸吗？重阳节快乐！

29、九九重阳节就要到了，提前祝愿所有活跃的、沉默的、热情的、深沉的、玩酷的、耍赖的、在线的、隐身的朋友们：重阳节快乐！

30、轻风拂山岗，遍野黄，佳节到重阳，思君登高望。情意心中藏，祝福暖心房，快乐似水长，好运伴君旁。鸿雁带福至，悠闲莫匆忙，天高云淡时，愿你永安康！

31、今又重阳，祝福送上，敬老踏秋，只为安康。登高眺望，前程光芒，轻嗅菊香，心情舒畅，糕点黄黄，幸福荡漾，九九节日，祝你全家快乐流长。

32、为了让你更加开心，为了让你忘记铰单，为了让你感受到生活的美好，请大声地念出一下的祝福语：重阳节快乐。

33、酒是什么样的酒，友情长青酒；久是什么样的久，天长又地久；九是什么样的九，一四六八九，祝你丰"一"足"四"，工作顺"六""八"大财。重阳快乐！

34、独在异乡过重阳，每逢佳节把你想，不知好友怎么样，编条短信送吉祥。过个重阳不容易，即使不能在一起，祝福收到笑嘻嘻：祝重阳节快乐重重！重阳节快乐！

35、双九重阳佳节到，微信祝福来问好：相逢总是很奇妙，生活无忧无烦恼，工作压力瞬间消，伤心失落全跑掉，锻炼身体多散步，愿你重阳更美好！

36、九月九，送美酒，桃花酒，姻缘到，甜蜜爱情香又香；幸福酒，好运旺，快乐人生乐安康；友谊酒，情意深，久久的祝福长又长；九月九，重阳到，祝你快乐久久，"久"是幸福哟！

37、恁个久没盯到你，重阳节我还是整条短信，假巴意思问候哈你嘛！祝愿你身体巴巴适适勒，生活得安安逸逸勒，工作得耍耍搭搭勒，日子过得撑撑抖抖勒！

38、您生命的秋天，是枫叶一般的色彩，不是春光胜似春光，时值霜天季节，却格外显得神采奕奕。祝您老重阳节健康长寿！

39、但是夕阳无限好，何须惆怅近黄昏！ 苍龙日暮还行雨，老树春深更着花！ 祝您越活越精神！年年重阳都快乐！

40、曾经您告诉我，三月三风筝飞上天，那是童年的快乐。现在，九月九香，是老人节，是您的节日，亲爱的爹娘，祝你们身体安康、福寿永享，重阳节快乐！

41、想念你，在每一个嫁接；祝福你，在每一个新的一天；愿快乐在你身边围绕，让美梦总是在你身边守候。重阳来临的时刻，愿你今天比昨天更快乐！

42、斟上一杯重阳酒，遥表思念牵挂长，身插茱萸祈平安，悠悠惦念在心间，又到一年重阳节，点点祝福随风传，重阳节，愿你平安幸福，重阳快乐！

43、愿你薪水多赚，奖金满满；快乐天天，幸福年年！祝我们这些表面风光内心彷徨；容颜未老，心已沧桑；成就难有，郁闷经常；比蚂蚁忙；比岳飞更忠良的兄弟姐妹们，重阳节快乐！

44、时光流逝，流不走我心中的你！将万千思念托付那吉祥鸟，带去我深深的问候；时间阻挡不了那份执著，不管未来之路多么崎岖，今天给你送上重阳祝福！祝你重阳节快乐！

45、一丝秋雨秋意浓，一缕秋风秋情动，一轮明月谁与共，一份思念遥相送，一枝茱萸情义重，一朵香入梦，一条微信你会懂，一片真情重阳祝福中。

46、心存明月，月照九州，可知人生本无常？正香，今夕昨昔相聚相离，琴瑟琵琶丝竹管弦，遍插茱萸，花中行乐月中眠，半醉半醒，谱写重阳喜庆的乐章

47、凋零的不是那季节，而是花朵；成熟的不是那果实，而是岁月；南飞的不是那大雁，而是心情；牵挂的不是那日子，而是家人。重阳节，气候变，请您注意健康！

48、九月九，重阳节，一样的季节，一样的日子，一样的牵挂，一样的思念，一样的深情，一样的心情，一样的问候，一样的祝福，愿你拥有一个愉快的重阳佳节！

49、重阳节到了，回忆起你我当初在一起的日子，那是一段永远难忘的友情岁月，当你在孤独时别忘记还有一位老朋友惦记着你，真心祝你一切顺利，天天开心。

50、岁岁重阳，今又重阳，九九相逢祥瑞降。不似春光，胜似春光，秋高气爽喜洋洋。金菊飘香，归雁成行，健康喜乐福绵长。重阳节，祝你幸福安康，和和美美！

文案二

1、秋风凉，秋菊香，秋景中迎来重阳，合家欢，兄弟聚，好日子里秉烛叙，插茱萸，齐登高，九九快乐更美好，思念深，祝福来，祝你重阳开心愉快！

2、重阳佳节忆兄弟，千言万语不足惜；有人登高去远眺，有人林中来采菊；我在这里想着你，愿你重阳好运气；幸福全都在心底，健康好梦更吉利！

3、重阳佳节团聚，亲朋好友团聚，情人恋人团聚，你我他团聚，在这个快乐的节日里！真心祝福你：事业丰收，薪水丰收，爱情丰收，快乐永相伴！

4、重阳佳节珍贵，周末不能浪费。出门旅游太累，登高望远太贵。回家孝敬父母，享受亲情安慰。尝尝妈妈手艺，美食值得回味。家庭和谐幸福，工作才不疲惫！

5、重阳佳节又来临，送你几条小建议，出游一定不要去，费钱费神又费力，登高危险系数大，一不小心就要上担架，不如发个短信，打个电话，陪我一起诉牵挂！

6、重阳将来到，快乐步步高。人间重晚晴，殷殷寸草心。霜叶红胜花，岁岁好年华。莫道桑榆晚，幸福金不换。愿你重阳享安康，吉祥伴身旁。

7、重阳佳节来相聚，亲朋好友在一起，情人恋人笑欢语，你我短信传祝福。让我们为明天美好的前程相聚吧！相聚重阳，相守未来，重阳快乐！

8、莫道重阳秋风凉，果实累累喜洋洋；莫道重阳雨霏霏，红叶挂满心头醉；莫道重阳夕阳红，阅尽风霜霞光美；莫道重阳话凄凉，登高远眺思绪飞！祝重阳节快乐！

9、飘香，秋风清凉，高望远，开心舒畅，遍插茱萸，互祝健康，重阳佳节，兄弟同堂，故人重逢，畅抒心房，亲情友情，随秋高扬，共度重阳，同庆安康。

10、重阳佳节到来，愿你心里幸福暖洋洋，开心笑声随风扬，美好心情任传扬，夫妻恩爱似鸳鸯，友情爱情齐荡漾，事业身体皆无恙，喜庆多多胜骄阳。

11、重阳佳节送你一条充满法力的短信，收到的人将会时来运转；前途平坦，兜里有款，甜长苦短，薪水翻番，好吃好穿，常有新欢，追求的路程越走越宽。

12、重阳佳节送你限量版重阳糕，祝你心情好开心程度高，工作顺办事效率高，薪水涨升级职位高，身体棒生活质量高，朋友多人气指数高，福气到运气与天齐高！

13、重阳佳节送你几颗心：早上出门舒心！路上小心！遇事耐心！做事细心！交友留心！待人诚心！对自己有信心！对情人有爱心！更重要的是重阳佳节你最开心！

14、重阳将来到，快乐步步高。人间重晚晴，殷殷寸草心。霜叶红胜花，岁岁好年华。莫道桑榆晚，幸福金不换。愿你重阳享安康，吉祥伴身旁。

15、岁岁重阳，今又重阳，九九相逢祥瑞降。不似春光，胜似春光，秋高气爽喜洋洋。金菊飘香，归雁成行，健康喜乐福绵长。重阳节，祝你幸福安康！

16、我愿化作一片云，随你漂流到天涯海角，我愿变成一首歌，寂寞时在你耳边响起，我用心编写最真挚的祝福，在这重阳佳节里送给您，愿您越活越精彩！

17、重阳佳节九月九，赠你酒，祝你平安健康久；邀你爬山观秋景，登高转运好运久；与你同享蜜花糕，事业甜蜜快乐久；送你一方茱萸佩，趋吉迎祥幸福久。

18、重阳佳节九月九，美好祝福送不够。生活甜蜜如美酒，爱情幸福到永久。工作轻松常袖手，家庭和谐福常有。诸事顺利烦恼走，一切如意喜临头。

19、思念如一叶扁舟，在岁月的长河中漂流，划过一年一年一天一天。记录点点滴滴，丝丝缕缕，到重阳节那天，一触即发！祝重阳节快乐！

20、九月九，再聚首，重阳相聚会老友；是亲友，是朋友，一切尽在杯中酒；情也深，爱也久，佳节团圆来叙旧；念今昔，盼永久，每年相聚九月九。重阳节快乐！

21、送你一盒重阳糕，它以祝福与问候为原料，相间叠放：一层幸福粉，一层平安糖，一层开心豆，一层健康果，愿你重阳节快乐。

22、重阳临，提前许九个美丽愿望送你：一愿心情好，二愿忧愁抛，三愿幸福绕，四愿收入高，五愿身体康，六愿烦恼消，七愿不变老，八愿困难少，九愿乐逍遥！

23、重阳佳节九月九，吉祥好运送朋友：事事顺心好运有，烦恼没有幸福留；亲情友情和爱情，犹如日月天长久；短信祝福送你手，美满如意到久久。重阳节快乐！

24、秋菊，倾吐悠悠清香；茱萸，环绕片片吉祥；登高，遥寄无限思念；酒杯，斟满情谊美酒；心灵，发送诚挚祝福：九九重阳，愿你开心，心情愉悦！

25、将彩云编织缱绻的梦境，收集心中每一份感动，许下星空每一个祝愿，记载心头每一丝企盼，交织成一首幸福美丽的乐章，在这重阳佳节伴随你！

26、祝福你理想幻想梦想心想事成；公事私事心事事事称心；财路运路人生路路路畅通；晴天雪天天天开心，亲情友情爱情情情似海；重阳节快乐！

27、重阳佳节秋高气爽，登高锻炼飘香，遥望远方思绪飞扬，家中亲人是否安好？发条短信道声平安，问声安好表表心愿，祝福亲友久久好运久久健康！

28、重阳佳节秋风凉，多添衣来保安康，时节好做茶，泻火安神心清爽，天渐干多多喝水，夜渐长作息如常，饮食注意规律，锻炼适当加强，愿重阳再添安康。

29、有你相伴，黄金不换，有我祝福，身体舒服，快乐重阳，天天吉祥，短信字少，情意却多，在这美好的节日里祝你永远幸福，重阳节快乐！

30、重阳节，登高行。鲜花旁，笑脸映。空气朗，伴清风。吉祥茶，如意饼。邀亲朋，相思情。若怀念，望荧屏。问候语，表心声。遥祝愿，笑不停。祝重阳快乐！

31、岁岁重阳，今又重阳，又见香；不是春光，胜似春光，秋色迷人心芬芳。让我登上高处，为你送来真诚和爱的祝福：祝重阳快乐，合家康泰！

32、岁岁重阳，今又重阳，谁道秋日凉？重阳节里歌声放，祝福飞来心飞扬。九月九里共举杯，声声祝福暖心扉。短信送到你这里，祝福也能化伤悲！

33、重阳节到了，也没啥好送你的，就送你一束吧。可别小看它，你可以用它酿成酒，饮后无忧愁；还可制成糕，吃后乐逍遥。重阳节快乐！

34、岁岁重阳，今又重阳，不是春光，胜似春光，一根扁担，两挑箩筐，三秋雁阵，四分五行，六六大顺，七下八上，九九重阳，十月农忙，短信在此，祝你健康！

35、虽然我不能陪你品酒赏菊，但我们可以共赏同一条短信；虽然我不能陪你登高插萸，但我们可以转发同一条祝福；重阳节，总有一条短信专门送给你。

36、重阳佳节翩然到，尊长敬老尽孝道，登高祈福好运临，家人同食重阳糕。金黄富贵临，茱萸悬门福气绕，踏秋田野赏美景，举杯美酒吉祥兆。愿你重阳心情好，家人团聚乐逍遥，健康常在迎祥瑞，福气临门好运到！

37、重阳佳节到来，亲朋好友开怀，祝你心情愉快，心事统统放开。九月九，让我们一起登高望远吧，借着醇香的美酒，分享心中的忧愁与快乐，重阳节快乐！

38、重阳佳节到来了，亲朋好友开怀笑，心情愉快外出游，心事统统来丢掉。九月初九好日子，登高望远吉祥拥，醇香美酒举杯饮，分享心中忧与乐。祝愿朋友重阳节快乐！

39、空气中弥漫着欢乐，树梢上飘落着祝福，重阳的温馨在招手，节日的激情在喷薄。偶愿化作清风、阳光、白云，给你载来如意、健康、财富。重阳节快乐重阳节来历！

40、带一颗心去登高，让一双眼去望远，携一份情怀去思念，寄一缕清风去相见，托一个好梦去连线，送一声祝福去你身边。重阳节，愿你步步高，天天好。

41、送一缕阳光给你，在秋日的萧瑟中为你捎去温暖，送一份祝福给你，在重阳佳节即将到来的日子里为你带来关怀，预祝重阳节快乐，天天快乐。

42、送上一支山茱萸，愿你永远愉快；送上一块重阳糕，愿你永远高兴；送上一杯酒，愿你幸福久久。重阳节到了，祝福你节日天天好心情！

43、送你清风让你心旷神怡，送你细雨洗去你疲惫的汗迹，让蓝天作封白云作信，让流星作我的特快专递，送你彩虹通向梦想之旅，祝你重阳节快乐！

44、重阳敬请歇一歇，又是一个传统节；南方飘雨北方雪，朋友还是我俩铁；人生相逢皆缘起，日月轮转点点滴；祝福就是送给你，祝你快乐又如意！

45、九九芳辰重阳鹤添寿，愿秋风捎去我的思念和祝福，祝你越活越精神，越活越年轻！

46、借此佳节之际我感谢你，你的笑颜似灿烂的阳光照亮了我的'世界，你给予我的安慰和鼓励支持我度过一切艰苦，言语不能表达我对你的情谊，你是一位了不起的人。祝你重阳节快乐！

47、江涵秋影雁初飞，与客携壶上翠微。尘世难逢开口笑，须插满头归。但将酩酊酬佳节，不作登临恨落晖。古往今来只如此，牛山何必独沾衣。

48、红颜未虚伏虎降龙，白发带笑铺金叠翠！天高气爽，人寿花香！